Serveur d'exploration SRAS

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein

Identifieur interne : 004257 ( Main/Exploration ); précédent : 004256; suivant : 004258

Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein

Auteurs : Chao Qian [République populaire de Chine] ; Di Qin [République populaire de Chine] ; Qiao Tang [République populaire de Chine] ; Yi Zeng [République populaire de Chine] ; Guixia Tang [République populaire de Chine] ; Chun Lu [République populaire de Chine]

Source :

RBID : ISTEX:83CEF669667DB4778C3943C5292BB7E06F425360

Descripteurs français

English descriptors

Abstract

Abstract: To identify the potential B-cell antigenic epitopes within the N-terminus of SARS-CoV (SARS-associated coronavirus, SARS-CoV) M protein and characterize monoclonal antibody (MAb) against the protein as well as its recognizing region, we expressed and purified a portion of SARS-CoV M protein (amino acid 1–43) in Escherichia coli (E. coli). By using Western blot and enzyme-linked immunosorbent assay (ELISA), we showed that the purified recombinant M protein could be recognized by four SARS-CoV-positive human sera even when those sera were 12,800-fold diluted. Furthermore, we characterized one representative IgG2 MAb, 3H9, which exhibited a strong immunoreaction to both recombinant M protein and native viral protein of SARS-CoV. We found a B-cell antigenic epitope located between amino acid 1–15 and defined the MAb recognizing region within amino acid 16–28 of M. These findings not only suggest that both recombinant M protein and its specific MAbs may be used as the diagnostic reagents for SARS, but also provide a potential target site for the design of an epitope-based vaccine against SARS.

Url:
DOI: 10.1007/s11262-005-0050-8


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

<record>
<TEI wicri:istexFullTextTei="biblStruct">
<teiHeader>
<fileDesc>
<titleStmt>
<title xml:lang="en">Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein</title>
<author>
<name sortKey="Qian, Chao" sort="Qian, Chao" uniqKey="Qian C" first="Chao" last="Qian">Chao Qian</name>
</author>
<author>
<name sortKey="Qin, Di" sort="Qin, Di" uniqKey="Qin D" first="Di" last="Qin">Di Qin</name>
</author>
<author>
<name sortKey="Tang, Qiao" sort="Tang, Qiao" uniqKey="Tang Q" first="Qiao" last="Tang">Qiao Tang</name>
</author>
<author>
<name sortKey="Zeng, Yi" sort="Zeng, Yi" uniqKey="Zeng Y" first="Yi" last="Zeng">Yi Zeng</name>
</author>
<author>
<name sortKey="Tang, Guixia" sort="Tang, Guixia" uniqKey="Tang G" first="Guixia" last="Tang">Guixia Tang</name>
</author>
<author>
<name sortKey="Lu, Chun" sort="Lu, Chun" uniqKey="Lu C" first="Chun" last="Lu">Chun Lu</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:83CEF669667DB4778C3943C5292BB7E06F425360</idno>
<date when="2006" year="2006">2006</date>
<idno type="doi">10.1007/s11262-005-0050-8</idno>
<idno type="url">https://api.istex.fr/ark:/67375/VQC-9QPS6TR4-7/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000123</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000123</idno>
<idno type="wicri:Area/Istex/Curation">000123</idno>
<idno type="wicri:Area/Istex/Checkpoint">001683</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001683</idno>
<idno type="wicri:doubleKey">0920-8569:2006:Qian C:identification:of:a</idno>
<idno type="wicri:source">PMC</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088559</idno>
<idno type="RBID">PMC:7088559</idno>
<idno type="wicri:Area/Pmc/Corpus">000504</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000504</idno>
<idno type="wicri:Area/Pmc/Curation">000504</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000504</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001114</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001114</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:16972028</idno>
<idno type="wicri:Area/PubMed/Corpus">002094</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002094</idno>
<idno type="wicri:Area/PubMed/Curation">002094</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002094</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002159</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002159</idno>
<idno type="wicri:Area/Ncbi/Merge">001653</idno>
<idno type="wicri:Area/Ncbi/Curation">001653</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001653</idno>
<idno type="wicri:doubleKey">0920-8569:2006:Qian C:identification:of:a</idno>
<idno type="wicri:Area/Main/Merge">004473</idno>
<idno type="wicri:Area/Main/Curation">004257</idno>
<idno type="wicri:Area/Main/Exploration">004257</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title level="a" type="main" xml:lang="en">Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein</title>
<author>
<name sortKey="Qian, Chao" sort="Qian, Chao" uniqKey="Qian C" first="Chao" last="Qian">Chao Qian</name>
<affiliation wicri:level="1">
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>The Laboratory of Molecular Virology, Jiangsu Province Laboratory of Pathogen Biology, Department of Microbiology and Immunology, Nanjing Medical University, 210029, Nanjing</wicri:regionArea>
<wicri:noRegion>Nanjing</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Qin, Di" sort="Qin, Di" uniqKey="Qin D" first="Di" last="Qin">Di Qin</name>
<affiliation wicri:level="1">
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>The Laboratory of Molecular Virology, Jiangsu Province Laboratory of Pathogen Biology, Department of Microbiology and Immunology, Nanjing Medical University, 210029, Nanjing</wicri:regionArea>
<wicri:noRegion>Nanjing</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Tang, Qiao" sort="Tang, Qiao" uniqKey="Tang Q" first="Qiao" last="Tang">Qiao Tang</name>
<affiliation wicri:level="1">
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>The Laboratory of Molecular Virology, Jiangsu Province Laboratory of Pathogen Biology, Department of Microbiology and Immunology, Nanjing Medical University, 210029, Nanjing</wicri:regionArea>
<wicri:noRegion>Nanjing</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Zeng, Yi" sort="Zeng, Yi" uniqKey="Zeng Y" first="Yi" last="Zeng">Yi Zeng</name>
<affiliation wicri:level="1">
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>The Laboratory of Molecular Virology, Jiangsu Province Laboratory of Pathogen Biology, Department of Microbiology and Immunology, Nanjing Medical University, 210029, Nanjing</wicri:regionArea>
<wicri:noRegion>Nanjing</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Tang, Guixia" sort="Tang, Guixia" uniqKey="Tang G" first="Guixia" last="Tang">Guixia Tang</name>
<affiliation wicri:level="1">
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>The Laboratory of Molecular Virology, Jiangsu Province Laboratory of Pathogen Biology, Department of Microbiology and Immunology, Nanjing Medical University, 210029, Nanjing</wicri:regionArea>
<wicri:noRegion>Nanjing</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Lu, Chun" sort="Lu, Chun" uniqKey="Lu C" first="Chun" last="Lu">Chun Lu</name>
<affiliation wicri:level="1">
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>The Laboratory of Molecular Virology, Jiangsu Province Laboratory of Pathogen Biology, Department of Microbiology and Immunology, Nanjing Medical University, 210029, Nanjing</wicri:regionArea>
<wicri:noRegion>Nanjing</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1">
<country wicri:rule="url">République populaire de Chine</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series>
<title level="j">Virus Genes</title>
<title level="j" type="abbrev">Virus Genes</title>
<idno type="ISSN">0920-8569</idno>
<idno type="eISSN">1572-994X</idno>
<imprint>
<publisher>Kluwer Academic Publishers</publisher>
<pubPlace>Boston</pubPlace>
<date type="published" when="2006-10-01">2006-10-01</date>
<biblScope unit="volume">33</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="147">147</biblScope>
<biblScope unit="page" to="156">156</biblScope>
</imprint>
<idno type="ISSN">0920-8569</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt>
<idno type="ISSN">0920-8569</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Adult</term>
<term>Animals</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antigens, Viral (chemistry)</term>
<term>Antigens, Viral (immunology)</term>
<term>B-cell antigenic epitope</term>
<term>Blotting, Western</term>
<term>ELISA</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Epitopes, B-Lymphocyte (immunology)</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Middle Aged</term>
<term>Monoclonal antibody</term>
<term>Recombinant Fusion Proteins (immunology)</term>
<term>Recombinant Fusion Proteins (isolation & purification)</term>
<term>Recombinant M protein</term>
<term>SARS Virus (immunology)</term>
<term>SARS-associated coronavirus</term>
<term>Severe Acute Respiratory Syndrome (diagnosis)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Viral Matrix Proteins (immunology)</term>
<term>Viral Matrix Proteins (isolation & purification)</term>
<term>Western blot</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Animaux</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Antigènes viraux ()</term>
<term>Antigènes viraux (immunologie)</term>
<term>Déterminants antigéniques des lymphocytes B (immunologie)</term>
<term>Humains</term>
<term>Protéines de fusion recombinantes (immunologie)</term>
<term>Protéines de fusion recombinantes (isolement et purification)</term>
<term>Protéines de la matrice virale (immunologie)</term>
<term>Protéines de la matrice virale (isolement et purification)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Syndrome respiratoire aigu sévère (diagnostic)</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en">
<term>Antigens, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en">
<term>Antibodies, Monoclonal</term>
<term>Antigens, Viral</term>
<term>Epitopes, B-Lymphocyte</term>
<term>Recombinant Fusion Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en">
<term>Recombinant Fusion Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en">
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr">
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr">
<term>Anticorps monoclonaux</term>
<term>Antigènes viraux</term>
<term>Déterminants antigéniques des lymphocytes B</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de la matrice virale</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en">
<term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr">
<term>Protéines de fusion recombinantes</term>
<term>Protéines de la matrice virale</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr">
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en">
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Adult</term>
<term>Animals</term>
<term>Blotting, Western</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Middle Aged</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Adulte</term>
<term>Adulte d'âge moyen</term>
<term>Animaux</term>
<term>Antigènes viraux</term>
<term>Humains</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Technique de Western</term>
<term>Test ELISA</term>
</keywords>
</textClass>
<langUsage>
<language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front>
<div type="abstract" xml:lang="en">Abstract: To identify the potential B-cell antigenic epitopes within the N-terminus of SARS-CoV (SARS-associated coronavirus, SARS-CoV) M protein and characterize monoclonal antibody (MAb) against the protein as well as its recognizing region, we expressed and purified a portion of SARS-CoV M protein (amino acid 1–43) in Escherichia coli (E. coli). By using Western blot and enzyme-linked immunosorbent assay (ELISA), we showed that the purified recombinant M protein could be recognized by four SARS-CoV-positive human sera even when those sera were 12,800-fold diluted. Furthermore, we characterized one representative IgG2 MAb, 3H9, which exhibited a strong immunoreaction to both recombinant M protein and native viral protein of SARS-CoV. We found a B-cell antigenic epitope located between amino acid 1–15 and defined the MAb recognizing region within amino acid 16–28 of M. These findings not only suggest that both recombinant M protein and its specific MAbs may be used as the diagnostic reagents for SARS, but also provide a potential target site for the design of an epitope-based vaccine against SARS.</div>
</front>
</TEI>
<affiliations>
<list>
<country>
<li>République populaire de Chine</li>
</country>
</list>
<tree>
<country name="République populaire de Chine">
<noRegion>
<name sortKey="Qian, Chao" sort="Qian, Chao" uniqKey="Qian C" first="Chao" last="Qian">Chao Qian</name>
</noRegion>
<name sortKey="Lu, Chun" sort="Lu, Chun" uniqKey="Lu C" first="Chun" last="Lu">Chun Lu</name>
<name sortKey="Lu, Chun" sort="Lu, Chun" uniqKey="Lu C" first="Chun" last="Lu">Chun Lu</name>
<name sortKey="Qin, Di" sort="Qin, Di" uniqKey="Qin D" first="Di" last="Qin">Di Qin</name>
<name sortKey="Tang, Guixia" sort="Tang, Guixia" uniqKey="Tang G" first="Guixia" last="Tang">Guixia Tang</name>
<name sortKey="Tang, Qiao" sort="Tang, Qiao" uniqKey="Tang Q" first="Qiao" last="Tang">Qiao Tang</name>
<name sortKey="Zeng, Yi" sort="Zeng, Yi" uniqKey="Zeng Y" first="Yi" last="Zeng">Yi Zeng</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004257 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004257 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:83CEF669667DB4778C3943C5292BB7E06F425360
   |texte=   Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein
}}

Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021