Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein
Identifieur interne : 004257 ( Main/Exploration ); précédent : 004256; suivant : 004258Identification of a B-cell antigenic epitope at the N-terminus of SARS-CoV M protein and characterization of monoclonal antibody against the protein
Auteurs : Chao Qian [République populaire de Chine] ; Di Qin [République populaire de Chine] ; Qiao Tang [République populaire de Chine] ; Yi Zeng [République populaire de Chine] ; Guixia Tang [République populaire de Chine] ; Chun Lu [République populaire de Chine]Source :
- Virus Genes [ 0920-8569 ] ; 2006-10-01.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Animaux, Anticorps monoclonaux (immunologie), Antigènes viraux (), Antigènes viraux (immunologie), Déterminants antigéniques des lymphocytes B (immunologie), Humains, Protéines de fusion recombinantes (immunologie), Protéines de fusion recombinantes (isolement et purification), Protéines de la matrice virale (immunologie), Protéines de la matrice virale (isolement et purification), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Technique de Western, Test ELISA, Virus du SRAS (immunologie).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Anticorps monoclonaux, Antigènes viraux, Déterminants antigéniques des lymphocytes B, Protéines de fusion recombinantes, Protéines de la matrice virale, Syndrome respiratoire aigu sévère, Virus du SRAS.
- isolement et purification : Protéines de fusion recombinantes, Protéines de la matrice virale.
- virologie : Syndrome respiratoire aigu sévère.
- Adulte, Adulte d'âge moyen, Animaux, Antigènes viraux, Humains, Souris, Souris de lignée BALB C, Technique de Western, Test ELISA.
English descriptors
- KwdEn :
- Adult, Animals, Antibodies, Monoclonal (immunology), Antigens, Viral (chemistry), Antigens, Viral (immunology), B-cell antigenic epitope, Blotting, Western, ELISA, Enzyme-Linked Immunosorbent Assay, Epitopes, B-Lymphocyte (immunology), Humans, Mice, Mice, Inbred BALB C, Middle Aged, Monoclonal antibody, Recombinant Fusion Proteins (immunology), Recombinant Fusion Proteins (isolation & purification), Recombinant M protein, SARS Virus (immunology), SARS-associated coronavirus, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Viral Matrix Proteins (immunology), Viral Matrix Proteins (isolation & purification), Western blot.
- MESH :
- chemical , chemistry : Antigens, Viral.
- chemical , immunology : Antibodies, Monoclonal, Antigens, Viral, Epitopes, B-Lymphocyte, Recombinant Fusion Proteins, Viral Matrix Proteins.
- chemical , isolation & purification : Recombinant Fusion Proteins, Viral Matrix Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Adult, Animals, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Mice, Inbred BALB C, Middle Aged.
Abstract
Abstract: To identify the potential B-cell antigenic epitopes within the N-terminus of SARS-CoV (SARS-associated coronavirus, SARS-CoV) M protein and characterize monoclonal antibody (MAb) against the protein as well as its recognizing region, we expressed and purified a portion of SARS-CoV M protein (amino acid 1–43) in Escherichia coli (E. coli). By using Western blot and enzyme-linked immunosorbent assay (ELISA), we showed that the purified recombinant M protein could be recognized by four SARS-CoV-positive human sera even when those sera were 12,800-fold diluted. Furthermore, we characterized one representative IgG2 MAb, 3H9, which exhibited a strong immunoreaction to both recombinant M protein and native viral protein of SARS-CoV. We found a B-cell antigenic epitope located between amino acid 1–15 and defined the MAb recognizing region within amino acid 16–28 of M. These findings not only suggest that both recombinant M protein and its specific MAbs may be used as the diagnostic reagents for SARS, but also provide a potential target site for the design of an epitope-based vaccine against SARS.
Url:
- https://api.istex.fr/ark:/67375/VQC-9QPS6TR4-7/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088559
DOI: 10.1007/s11262-005-0050-8
Affiliations:
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Le document en format XML
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<term>Recombinant Fusion Proteins</term>
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<front><div type="abstract" xml:lang="en">Abstract: To identify the potential B-cell antigenic epitopes within the N-terminus of SARS-CoV (SARS-associated coronavirus, SARS-CoV) M protein and characterize monoclonal antibody (MAb) against the protein as well as its recognizing region, we expressed and purified a portion of SARS-CoV M protein (amino acid 1–43) in Escherichia coli (E. coli). By using Western blot and enzyme-linked immunosorbent assay (ELISA), we showed that the purified recombinant M protein could be recognized by four SARS-CoV-positive human sera even when those sera were 12,800-fold diluted. Furthermore, we characterized one representative IgG2 MAb, 3H9, which exhibited a strong immunoreaction to both recombinant M protein and native viral protein of SARS-CoV. We found a B-cell antigenic epitope located between amino acid 1–15 and defined the MAb recognizing region within amino acid 16–28 of M. These findings not only suggest that both recombinant M protein and its specific MAbs may be used as the diagnostic reagents for SARS, but also provide a potential target site for the design of an epitope-based vaccine against SARS.</div>
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<name sortKey="Tang, Guixia" sort="Tang, Guixia" uniqKey="Tang G" first="Guixia" last="Tang">Guixia Tang</name>
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<name sortKey="Zeng, Yi" sort="Zeng, Yi" uniqKey="Zeng Y" first="Yi" last="Zeng">Yi Zeng</name>
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